利用基因工程大肠杆菌从葡萄糖制备4-乙烯基苯酚的研究
摘 要
近年来，人们对芳香族天然产物生物合成途径的认识不断提高，代谢工程和合成生物学的迅速发展，是芳香族天然产物及其衍生物在微生物中异源生物合成的一种很有前途的选择。
- 乙烯基苯酚，也称为对羟基苯乙烯，为酚酸类物质的4-乙烯基衍生物，具有挥发性，有独特的气味，嗅觉识别度较高。4-乙烯基苯酚具有苯酚，药物和辛香风味，可用于食品添加剂及香精的配制，是光敏电阻，光刻和半导体制造的主要成分。它作为一种单体可用于合成聚 4-乙烯基苯酚，作为一种石油基原料用于树脂，橡胶，粘合剂的合成。本实验通过对香豆酸的脱羧作用，使用酪氨酸解氨酶和酚酸脱羧酶在大肠杆菌中实现从葡萄糖到对4-乙烯基苯酚的生物合成。之后对两种酶的诱导时间、诱导温度、IPTG浓度等条件进行优化实验，最终选择诱导温度25 ℃、诱导时间12 h、IPTG浓度0.4 mM、分子伴侣pKJE7、分子伴侣诱导剂L-Ara浓度0.5 mg/mL为产物表达的最佳条件。
关键词：生物合成、芳香天然产物、4-乙烯基苯酚、条件优化。
Preparation of 4-vinylphenol from glucose by genetically engineered Escherichia coli
ABSTRACT
In recent years, the understanding of the biosynthesis pathway of aromatic natural products has been improved, and the rapid development of metabolic engineering and synthetic biology, It is a promising choice for heterologous biosynthesis of aromatic natural products and their derivatives in microorganisms. However, the mass production of these compounds in the industrial environment has not yet been determined.
4-vinylphenol, also known as p-hydroxystyrene, is a 4-vinyl derivative of phenolic acids, which is volatile, has a unique odor, and has a high olfactory recognition of 4-vinylphenol with phenol, drugs and aroma flavor, 4-vinylphenol, 4-vinylphenol, also known as p-hydroxystyrene, is a 4-vinyl derivative of phenolic acids. Can be used in food additives and flavor preparation, is Guang Min resistance, photolithography and semiconductor manufacturing of the main components. It can be used as a monomer for the synthesis of poly (4-vinylphenol) and as a petroleum-based raw material for the synthesis of resins, rubber and adhesives. By decarboxylation of coumaric acid, the biosynthesis of 4-vinylphenol from glucose to 4-vinylphenol in Escherichia coli was achieved by using tyrosine deaminase and phenol decarboxylase. The expression conditions of product 4-vinylphenol were optimized, including induction time, induction temperature, IPTG concentration and so on. Finally, the induction temperature was 25 ℃, the induction time was 12 h, the concentration of pKJE7, was 0.4 mm, and the molecular chaperone was pKJE7,. The concentration of molecular chaperone inducer 0.5mg/m was the best condition for the expression of the product.
Key words: Aromatic natural product, 4-vinyl phenol, Optimization.
以上是毕业论文文献综述，课题毕业论文、任务书、外文翻译、程序设计、图纸设计等资料可联系客服协助查找。
